NGS technologies have been evolving over the past 10 years, leading to substantial improvements in quality and yield however, certain approaches have proven to be more effective and adaptable than others. By contrast, long-read approaches provide read lengths that are well suited for de novo genome assembly applications and full-length isoform sequencing. Short-read sequencing approaches provide lower-cost, higher-accuracy data that are useful for population-level research and clinical variant discovery. There are two major paradigms in next-generation sequencing (NGS) technology: short-read sequencing and long-read sequencing.
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